What is R6K?

R6K is a self-transmissible plasmid (Kontomichalou et al., 1970).

What is ori biology?

The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive within cells. The replicons of plasmids are generally different from the those used to replicate the host’s chromosomal DNA, but they still rely on the host machinery to make additional copies.

What is ColE1 origin?

ColE1 is a plasmid found in bacteria. Its name derives from the fact that it carries a gene for colicin E1 (the cea gene). It also codes for immunity from this product with the imm gene. In addition, the plasmid has a series of mobility (mob) genes.

What is the purpose of the origin of replication?

The origin of replication determines the vector copy number, which could typically be in the range of 25–50 copies/cell if the expression vector is derived from the low-copy-number plasmid pBR322, or between 150 and 200 copies/cell if derived from the high-copy-number plasmid pUC.

What is a replicon in DNA?

A replicon was conceptualized as a unit of replication that consisted of a DNA sequence called the replicator (now called origin of replication or ori) and an initiator, a protein that interacts with the ori to initiate the replication bubble.

How many number of replicon is found in E coli?

A total of 1,015 E. coli isolates were examined for the presence of the 18 plasmid replicon types using the simplified three-panel multiplex PCR protocol.

What is the ori site?

Ori sites also called as ‘Site for Origin of Replication’ are found in both prokaryotic and eukaryotic DNA. These are the sites where DNA replication begins.

What do Colicins do?

Colicins exert their lethal action by first binding to specific receptors, which are outer membrane proteins used for the entry of specific nutrients. They are then translocated through the outer membrane and transit through the periplasm by either the Tol or the TonB system.