What does capillary electrophoresis detect?
What does capillary electrophoresis detect?
Capillary electrophoresis (CE) is the primary methodology used for separating and detecting short tandem repeat (STR) alleles in forensic DNA laboratories worldwide. This chapter examines the general principles and components of injection, separation, and detection of STR alleles using CE.
What is microfluidic capillary electrophoresis?
Microchip capillary electrophoresis (µCE) is a high-speed separation and analysis technology for various chemical components in complex samples in the chip microchannels made of glass, quartz, silicon, plastic, and other materials.
What is the principle of capillary electrophoresis?
The Capillary Zone Electrophoresis (CZE) is the most widely used mode due to its simplicity. CZE allows the analysis of ionized or ionizable compounds. Analytes are simply separated according to their charge/hydrodynamic radius ratio and migrate towards anode or cathode according to their charges.
What do multiple bands in gel electrophoresis represent?
Bands are the horizontal “bars” which are actually stained DNA molecules embedded in the gel. As the DNA molecules migrate through the gel, they are sorted according to their molecular weight, so that each band represents DNA of a specific molecular weight.
Why capillary electrophoresis is done?
Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation. It is a useful technique because there is a large range of detection methods available.
What are 2 main advantages of using capillary electrophoresis?
Capillary electrophoresis (CE) provides a number of advantages for analysts, including high separation efficiency, short analysis times, low waste generation, and a diverse range of applications.
What is electroosmotic flow in capillary electrophoresis?
Electroosmotic flow is observed when an electric field is applied to a solution in a capillary that has fixed charges on its interior wall. Charge is accumulated on the inner surface of a capillary when a buffer solution is placed inside the capillary.
What is microchip electrophoresis?
Definition: Microchip electrophoresis (MCE) is a miniaturized form of capillary electrophoresis. Electrophoresis is a common technique to separate macromolecules such as nucleic acids (DNA, RNA) and proteins.
What are the components of capillary electrophoresis?
A typical capillary electrophoresis system consists of a high-voltage power supply, a sample introduction system, a capillary tube, a detector and an output device. Some instruments include a temperature control device to ensure reproducible results.
Why are some bands darker in gel electrophoresis?
More DNA in a band gives more intense staining of that band. So, for example, 50ng of DNA in a band gives two times more (= brighter) staining than 25ng. You can see this very clearly in lane 7, where restriction fragments originating from one microgram of identical DNA molecules are separated.
