What is the principle of Spectrofluorimetry?

Fluorescence spectroscopy exploits the phenomenon of electron excitation upon collision with high energy particles like photons and other excited electrons and emission of photons while lowering their energy down to the ground state. The molecules showing the fluorescence activity are termed as fluorophores.

What are the application of fluorimetry?

Colorimeters are used for a wide variety of applications in the chemical and biological fields including, but not limited to, analysis of blood, water, soil nutrients and foodstuffs, determination of solution concentration, determination of reaction levels, determination of bacterial crop growth.

What is meant by Spectrofluorometer?

: a device for measuring and recording fluorescence spectra.

What are the advantages and disadvantages of molecular Spectrofluorometry?

Advantages and Disadvantages As it was already pointed, one of the most important advantages of this technique is due to its high sensitivity and specifity. Another is its fast and rapid diagnosis ability. The main disadvantage is that not all compounds fluoresce.

What is the difference between spectrophotometer and spectrofluorometer?

The key difference between spectrophotometer and spectrofluorometer is that spectrophotometer involves the measurement of absorption, whereas spectrofluorometer involves the transition of polyatomic fluorescent molecules from their higher energy level to a ground state by lowering their energy level through emission of …

What are the components of spectrofluorometer?

The essential components of a spectrofluorometer are a light source, an excitation monochromator, a sample cell/cuvette, an emission monochromator and a detector. The light source sends out light at the excitation wavelength of an analyte in a sample.

Why does pH affect fluorescence?

pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P.