What is the principle of GLC chromatography?

Principle. GLC is based upon partitioning of compounds between stationary liquid and mobile gas phase. Due to its high sensitivity, reproducibility, and speed of resolution, it is widely used for several qualitative and quantitative analyses.

What are the 4 types of column chromatography?

Column chromatography is therefore classified according to the type of fluid flow system used:

  • Gravity chromatography.
  • Low-pressure chromatography.
  • Medium-pressure chromatography (including fast protein liquid chromatography)
  • High-pressure/high-performance liquid chromatography (HPLC)

What are the three types of column chromatography?

There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange.

How is Chromotography used?

Chromatography can be used as an analytical tool, feeding its output into a detector that reads the contents of the mixture. It can also be used as a purification tool, separating the components of a mixture for use in other experiments or procedures.

What type of chromatography is GLC?

Gas chromatography—also referred to as gas-liquid chromatography (GLC)—is a specific type of chromatography that utilizes an inert gaseous mobile phase and a liquid stationary phase.

What is GLC in analytical chemistry?

Capillary gas–liquid chromatography (GLC) combined with mass spectrometry (MS) has been successfully used for the separation of complex mixtures of alkaloids. The aim of Gas–liquid chromatography–mass spectrometry (GLC/MS) is to operate both a gas chromatograph and a mass spectrometer.

What type of column is used in HPLC?

HPLC columns are mostly four main types Normal phase, Reverse Phase, and Ion Exchange columns. In the pharmaceutical industry mostly reverse columns are used for analysis..

Is silica polar or non polar?

polar
Silica gel is a polar adsorbent. This allows it to preferentially adsorb other polar materials. When it comes to polarity, materials interact more with like materials. This principle is particularly important to many laboratories, which use silica gel as the stationary phase for column chromatography separations.

What is an Rf value?

In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).

What is the full form of GLC?

GLC – Gas liquid chromatography.

What is the difference between gas liquid and gas solid chromatography?

The key difference between gas solid chromatography and gas liquid chromatography is that, in gas solid chromatography, the stationary phase is in the solid state whereas, in gas liquid chromatography, the stationary phase is in the liquid state.

What is the concept behind column chromatography?

The concept behind column chromatography is adsorption. In the mobile phase, a mixture of components is dissolved and introduced into the column and the components move depending upon their relative accordance.

How to increase the productivity of column chromatography?

Fractions can be collected automatically by means of fraction collectors. The productivity of chromatography can be increased by running several columns at a time. In this case multi stream collectors are used.

What are the stationary phases used in column chromatography?

Various stationary phases, such as silica, alumina, calcium phosphate, calcium carbonate, starch, and magnesia, and different solvent compositions based on the nature of compounds to be separated and isolated, are used in column chromatography.

What is the dry method of column chromatography?

For the dry method, the column is first filled with dry stationary phase powder, followed by the addition of mobile phase, which is flushed through the column until it is completely wet, and from this point is never allowed to run dry.