What is HPTLC principle?

Principle of HPTLC have similar approach and employ the same physical principles of TLC (adsorption chromatography) i.e. the principleof separation is adsorption. The mobile phase solvent flows through because of capillary action. The components move according to their affinities towards the adsorbent.

What is HPTLC and its application?

HPTLC is used for purity control of chemicals, pesticides, steroids, and water analysis. [50] HPTLC is also widely used for analysis of vitamins, water-soluble food dyes, pesticides in fruits, vegetables, and other food stuffs.

What are the features of HPTLC?

Important features of HPTLC are the ability to analyze cruder samples containing multicomponents; application of large number of sample and a series of standards using the spray-on technique; choice of solvents for the HPTLC development is wide as the mobile phases are fully evaporated before the detection step; …

What are the steps involved in HPTLC?

The HPTLC suite is composed of a number of components.

  • Sample Applicator – Application of sample to stationary phase i.e. TLC/HPTLC plate.
  • Development Chamber – Separation of components.
  • Derivatizer set and Plate heater – Derivatization: Chemical spraying of plate to detect compounds not visible under white/UV light.

What is the principle of HPTLC Mcq?

Principle :- The principle of separation is adsorption. When a mixture containing one or more components are spotted on a TLC of absorbant coated on a chromatographic plate and introduce into a m.p. which are continuing distributing. The m.p. is flow by capillary action.

What is HPLC and HPTLC?

Definition. HPLC refers to a form of liquid chromatography to separate compounds dissolved in a solution, while HPTLC refers to a most advanced form of planar chromatography.

Which lamps are used in HPTLC?

TLC Scanner III:With three light sources-mercury vapour lamp, deuterium lamp, or tungsten. HPTLC is a versatile separation technique and is official in the most of the pharmacopoeias for determining content uniformity, purity profile, assay values and dissolution rates in unlimited number of monographs.

What is the most popular application of HPTLC?

HPTLC – Speciality Chemicals Analysis HPTLC can be used very effectively in reverse engineering because it enables rapid comparison with e.g. standard provided by a buyer or other similar products in the market. Chemical reactions can be studied very quickly e.g. within 2 hours.

What type of chromatography is HPTLC?

HPTLC (high-performance thin layer chromatography) is a sophisticated form of TLC, which provides superior separation efficiency. The HPTLC concept includes validated methods for qualitative and quantitative analysis, and fulfills all quality requirements for use in fully regulated environments.

What is the principle of separation in TLC?

The separation principle of the TLC procedure is based on the given compound’s relative affinity towards the mobile and the stationary phase. The process begins here by moving the mobile phase over the stationary phase’s surface.

What is retardation value?

The Rf (retardation factor) value is the ratio of the solute’s distance travelled to the solvent’s distance travelled. The word comes from chromatography, when it was discovered that a given component will always travel the same distance in a given solvent under the same conditions.

What is difference between TLC and HPTLC?

TLC and HPTLC are two chemical techniques used to separate non-volatile components in a mixture. The key difference between TLC and HPTLC is that the absorbent material in TLC plate has large particles whereas HPTLC plates have very small particles of adsorbent material. TLC is thin layer chromatography.