What is domain in UniProt?

Last modified December 11, 2018. This subsection of the Family and Domains section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.

How do you get the amino acid sequence from UniProt?

Retrieving sequences from the website

  1. Perform your favorite query and view the resulting list of entries (e.g. this query retrieves all UniProtKB entries that are part of the human proteome: proteome:UP000005640)
  2. Click the Download button in the query result page.

Is UniProt a secondary database?

Hybrid databases and families of databases Many data resources have both primary and secondary characteristics. For example, UniProt accepts primary sequences derived from peptide sequencing experiments.

Who developed UniProt?

UniProt is a collaboration between the European Bioinformatics Institute (EMBL-EBI), the SIB Swiss Institute of Bioinformatics and the Protein Information Resource (PIR). Across the three institutes more than 100 people are involved through different tasks such as database curation, software development and support.

How do you find the domain in bioinformatics?

One way to identify a domain is to find the part of a target protein that has sequence or structural similarities with a template through homology alignment. Another way is to predict the domain boundaries from a protein sequence.

What are topological domains?

Topologically associating domains (TADs) are fundamental units of three-dimensional (3D) nuclear organization. The regions bordering TADs-TAD boundaries-contribute to the regulation of gene expression by restricting interactions of cis-regulatory sequences to their target genes.

How many sequences does UniProt contain?

The UniProt knowledgebase is a large resource of protein sequences and associated detailed annotation. The database contains over 60 million sequences, of which over half a million sequences have been curated by experts who critically review experimental and predicted data for each protein.

How do you get the protein structure from UniProt?

How can I retrieve all UniProtKB entries for which the 3D structure is known?

  1. Select Search in: Protein Knowledgebase (UniProtKB)
  2. Click Advanced Search ยป to open the query builder.
  3. Select Field: Cross-reference [DR]
  4. Select Database: PDB.
  5. Click Add & Search.

What kind of database is UniProt?

The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. The UniProt databases are the UniProt Knowledgebase (UniProtKB), the UniProt Reference Clusters (UniRef), and the UniProt Archive (UniParc).