What is cytotoxicity assay?

Cytotoxicity assays measure the ability of cytotoxic compounds to cause cell damage or cell death. Cytotoxicity assays are widely used in fundamental research and drug discovery to screen libraries for toxic compounds.

Is resazurin toxic to cells?

Resazurin, introduced as a cell viability indicator under the trade name alamarBlue(®), is generally regarded as nontoxic when used according to manufacturer’s suggested shorter-term incubation time specifications. However, problems arise when exposure times are extended to longer-term cultures on the order of days.

How is cytotoxicity measured in cells?

While it can be measured in a number of different ways, assessing cell viability through the use of vital dyes (formazan dyes), protease biomarkers or by measuring ATP content are some of the most commonly used methods in determining cytotoxicity.

Why is cytotoxicity assay performed?

Cytotoxicity assays measure loss of some cellular or intercellular structure and/or functions, including lethal cytotoxicity. They thus give an indication of the potential to cause cell and tissue injury and as such have been used by some investigators to predict tissue injury, including eye injury.

What is the meaning of cytotoxicity?

Listen to pronunciation. (SY-toh-TOK-sik AY-jent) A substance that kills cells, including cancer cells. These agents may stop cancer cells from dividing and growing and may cause tumors to shrink in size.

What is cytotoxicity of cell?

The effect of being toxic to cells caused by toxic agents is called cytotoxicity. Exposing cells to a cytotoxic compound may result in various outcomes in the cell. At this point, the cells may actively progress into the death phase.

What causes cytotoxicity?

Cytotoxicity is defined as the toxicity caused due to the action of chemotherapeutic agents on living cells.

Why cytotoxicity assay is done?