How does absorbance plate reader work?

A microplate reader detects light signals produced by samples which have been pipetted into a microplate. The optical properties of these samples are the result of a biological, chemical, biochemical or physical reaction. Different analytic reactions result in different optical changes used for analysis.

What is a BioTek plate reader?

RUO. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. The color touch screen provides a visual user interface, making programming fast and intuitive.

What is the difference between spectrophotometer and plate reader?

A microplate reader is essentially a spectrophotometer but with a few key differences. While a spectrophotometer works with a monochromator or filter-based optics to measure events in samples just like a microtiter plate reader, a spectrophotometer works with a single small sample size.

What is the name of the instrument used to measure absorbance?

Absorbance is measured using a spectrophotometer or microplate reader, which is an instrument that shines light of a specified wavelength through a sample and measures the amount of light that the sample absorbs.

How do Elisa plate readers work?

ELISA Plate Reader / Microplate Readers / Assay Readers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization.

Is ELISA reader a spectrophotometer?

The ELISA plate reader measures the absorbance of the solution in each well (cuvette) by recording the light passing through the solution from the top to the bottom of each well. The assay’s color, when tested in a standard spectrophotometer, is linear to an absorbance of 2.3.

Is Elisa reader a spectrophotometer?

How do you read absorbance readings?

Interpret the absorbance value. Absorbance can range from 0 to infinity such that an absorbance of 0 means the material does not absorb any light, an absorbance of 1 means the material absorbs 90 percent of the light, an absorbance of 2 means the material absorbs 99 percent of the light and so on.