What is a polony DNA sequencing?
What is a polony DNA sequencing?
Polony sequencing is an inexpensive but highly accurate multiplex sequencing technique that can be used to “read” millions of immobilized DNA sequences in parallel. This technique was first developed by Dr. George Church’s group at Harvard Medical School.
What is transcriptomic sequencing?
The set of genes which are transcribed in any one condition is known as the transcriptome, and the process of determining the genetic codes contained in the transcriptome, and their relative proportions, is known as transcriptome sequencing.
How does polony sequencing work?
1 General procedure for polony sequencing. A paired-tag genomic library is used as template for emulsion PCR on microbeads to generate polymerase colonies (polonies). The beads are cast on a coverslip in a thin layer of polyacrylamide, and put through iterative cycles of single-base sequencing.
What is a polony bioinformatics?
Polony is a contraction of “polymerase colony,” a small colony of DNA. Polonies are discrete clonal amplifications of a single DNA molecule, grown in a gel matrix. This approach greatly improves the signal-to-noise ratio.
What is the difference between exome and transcriptome?
The main difference between exome and transcriptome is that the exome is the complete sequence of all exons in protein-coding genes in the genome whereas the transcriptome is the collection of messenger RNA molecules derived from protein-coding genes.
What is the difference between PCR and Sanger sequencing?
the main difference between pcr and sanger sequencing is that pcr has 2 primers facing towards each other but sequencing has only one primer reading the sequence in one direction only.
Is Sanger more accurate than NGS?
NGS is significantly cheaper, quicker, needs significantly less DNA and is more accurate and reliable than Sanger sequencing. Let us look at this more closely. For Sanger sequencing, a large amount of template DNA is needed for each read.
