What does lactacystin do?

Lactacystin inhibits basic cell proliferation, its antineoplastic activity being dependent on inhibition of the UPS, which controls cellular protein turnover [6]. In Neuro-2a cells, lactacystin induces neurite outgrowth, a transient rise in cAMP levels and bipolar morphology of the cells.

Is MG132 reversible?

MG132 is a potent, reversible, and cell-permeable proteasome inhibitor (Ki = 4 nM). It belongs to the class of synthetic peptide aldehydes.

What does a proteasome inhibitor do?

Proteasome inhibitors are a type of drug that prevents proteasomes, the garbage disposal system of the cell, from chewing up excess proteins. The proteins build up and kill the myeloma cells.

How does MG132 inhibit the proteasome?

In the present work, we showed that the proteasome inhibitor MG132 interfered with the NFκB canonical pathway of TNFα-treated C2C12 cells by protecting IκBα from degradation and thus preventing the activation of NFκB transcription.

What does MG132 stand for?

MG132 (carbobenzoxy-Leu-Leu-leucinal) is a peptide aldehyde, which effectively blocks the proteolytic activity of the 26S proteasome complex.

What is the function of proteasomes?

The proteasome is a multisubunit enzyme complex that plays a central role in the regulation of proteins that control cell-cycle progression and apoptosis, and has therefore become an important target for anticancer therapy.

How does the proteasome work?

Proteasomes are protein complexes which degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. Enzymes that help such reactions are called proteases.

What are the functions of proteasomes?

The primary function of the proteasome is to degrade proteins (1). Proteasome substrates include signaling molecules, tumor suppressors, cell-cycle regulators, transcription factors, inhibitory molecules (whose degradation activate other proteins), and anti-apoptotic proteins (e.g., Bcl-2), among others (1).

Is proteasome an enzyme?

What is cycloheximide chase assay?

Cycloheximide chase assays are an experimental technique used in molecular and cellular biology to measure steady state protein stability. Cycloheximide is a drug that inhibits the elongation step in eukaryotic protein translation, thereby preventing protein synthesis.