What is the extinction coefficient of p-nitrophenol?

The extinction coefficient (ε) of p-nitrophenol was determined to be 15,000 M−1cm−1 under the assay conditions.

How do you calculate molar extinction coefficient?

According to Beer’s law, A = εbc, where A is the absorbance, ε is the molar extinction coefficient, b is the path length of the cuvette and c is the concentration. Thus, the molar extinction coefficient can be obtained by calculating the slope of the absorbance vs. concentration plot.

What is the molar absorptivity of p-nitrophenol?

The molar absorptivity of 4-nitrophenol (35 mumol/L) IN 10 mmol/L NaOH at 25 degrees C at 401 nm is 18380 +/- 90 L.

What is the molar extinction coefficient of PNP?

The accepted extinction coefficient for PNP is 1.7 x 104 M-1cm-1.

What is the extinction coefficient of PNP at 400 nm?

21,000 M-l-cm -1
The molar extinction coefficient of p-nitrophenol measured at 400 nm was 21,000 M-l-cm -1.

How do you find the concentration of p-nitrophenol?

The optimum pHs for acid and alkaline phosphatases are 5.3 and 10.5 respectively. Standard Solution: p-nitrophenol solution 0.5 mM, prepared in above buffer. Calculation: 1mM = 139 mg/l or 0.5mM = 69.5mg/l or 34.75 mg/500ml buffer.

What is molar extinction coefficient chemistry?

molar extinction coefficient. The term molar extinction coefficient (ε) is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength. It is an intrinsic property of chemical species that is dependent upon their chemical composition and structure.

How do you find the extinction coefficient of an amino acid sequence?

Absorbance and Extinction Coefficients Aλ = εcL = εc, when L = 1cm and where c is the protein concentration. where n is the number of each residue and the stated values are the amino acid molar absorptivities at 280nm and εmolar is molar extinction coefficient.

What is the extinction coefficient of alkaline phosphatase?

Extinction coefficient: E278 = 7.2 (Anderson et al. 1975). Isoelectric point: pH 4.5 (Garen and Levinthal 1960).

How do you prepare p-nitrophenol standard curve?

Standard curve was prepared by adding 10-100 μl of 1mM p-nitrophenol to 600 μl substrate buffer (0.1% Triton X-100 and 5 mM MgCl2, pH 10.4) and the volume was made up to 700 μL with 1% Triton X-100. To this reaction, 300 μL of 6M KOH was added.

What is p-nitrophenol used for in spectrophotometry?

The acyl esters of p-nitrophenol are the most commonly used model substrates to study the esterolytic activity of hydrolases. A quantitative detection of the released p-nitrophenolate can be easily carried out at 405–410 nm by spectrophotometry (Schmidt & Bornscheuer, 2005).

What is the molar attenuation coefficient ε?

Molar attenuation coefficient (molar absorptivity; molar extinction coefficient): The degree to which a solution absorbs light, in terms of the solution’s concentration. Represented by ε in the Beer-Lambert law. Greater ε = more light absorbed per mole of solute.