Is buffer solution required for PCR?

An optimal buffer system is essential to perform successful PCR. Reliable PCR results depend on many factors: the quality of the DNA and primers as well as the PCR instrument itself. Ampliqon has developed different Tris-based buffer solutions to meet different requirements in PCR applications.

Which buffer is used in RT PCR?

All commercially available real-time PCR buffers contain detergents (Triton X-100 or Tween 20) to enhance fluorescent dye and Taq DNA polymerase solubility.

What does the salt buffer do in PCR?

The KCl salt in the PCR buffer acts by neutralizing the charge present on the backbone of DNA. During the elongation step of the PCR, the primer has to anneal or stick properly to the template and this is facilitated by the KCl.

What does reaction buffer do?

A buffer is a solution that can resist pH change upon the addition of an acidic or basic components. It is able to neutralize small amounts of added acid or base, thus maintaining the pH of the solution relatively stable. This is important for processes and/or reactions which require specific and stable pH ranges.

What is RT buffer?

Reverse Transcription Buffer (10X) useful for reverse transcription reactions and production of cDNA, works with AMV Reverse Transcriptase and Taq DNA polymerase. Buffer is compatible with down stream applications such as PCR. Custom bulk orders are available upon request.

What is a buffer acid base?

A mixture of a weak acid and its conjugate base (or a mixture of a weak base and its conjugate acid) is called a buffer solution, or a buffer. Buffer solutions resist a change in pH when small amounts of a strong acid or a strong base are added (Figure 7.1. 1).

Why is Tris-HCl used in PCR?

Tris-HCl with pH 8.0 maintains the stability of the reaction and constant pH throughout the PCR reaction. This prevents the degradation of the DNA molecule.

What is standard buffer solution?

Standard Buffer Solutions are solutions of standard pH. They are used for reference purposes in pH measurements and for carrying out many pharmacopoeial tests which require adjustments to or maintenance of a specified pH.