What does T7 polymerase bind to?

promoter DNA sequence
T7 RNA polymerase initiates RNA synthesis after binding to a specific promoter DNA sequence and opening the DNA duplex; the enzyme remains bound to the promoter sequence throughout the initiation phase which involves the synthesis of an 8 to 12 nucleotide transcript.

What is the sequence of T7 promoter?

Bacteriophage T7 promoters contain a consensus sequence from -17 to +6 relative to the transcription start site, +1.

Which enzyme binds to the T7 promoter?

The polymerase efficiently and specifically transcribes genes bearing a T7 promoter (PT7). In the absence of T7 RNAP, the promoter does not direct transcription by endogenous polymerases in E. coli (Basu and Maitra, 1986).

What is the T7 terminator sequence?

The late bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3′ G residue just downstream of the U run.

Does the T7 promoter get transcribed?

T7 RNA polymerase starts transcription at the underlined G in the promoter sequence. The polymerase then transcribes using the opposite strand as a template from 5′->3′.

How are T7 genes transcribed?

c. T7 has its own RNA polymerase, which is packaged in its capsid and injected into the host during infection to transcribe T7 genes.

What is the T7 tag for?

The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

How does the T7 expression system work?

T7 expression hosts such as DE3 prophage strains or T7 Express strains carry a chromosomal copy of the phage T7 RNA polymerase gene. When inducer is added, T7 RNA polymerase is expressed and becomes dedicated to target gene transcription.

What’s a terminator sequence?

A sequence in DNA that signals termination of transcription to RNA Polymerase. This should not be confused with terminator codons that are the stopping signal for translation. Also known as: terminator, rho-independent termination site.

Is T7 promoter a strong promoter?

T7 RNA polymerase is very selective and efficient, resulting both in a high frequency of transcription initiation and effective elongation. These features result in an RNA elongation that is approximately five-fold faster than for E. coli RNA polymerase; hence, the T7 promoter is a much stronger promoter than the E.