What is the principle of the reticulin stain?

Reticulin Stain Kit is a metal impregnation technique where ammoniacal silver initially binds to the tissue component of interest. Then the reducing agent (formalin) produces a dark insoluble precipitate, enabling silver to be reduced into a visible state.

How do you prepare reticulin stain?

Method

  1. Deparaffinise sections with xylene then take through alcohols to water.
  2. Oxidise in acidified potassium permanganate for 3 minutes.
  3. Rinse in distilled water.
  4. Decolourise with 2% oxalic acid for 1 min.
  5. Rinse in distilled water.
  6. Mordant in 4% iron alum for 10 minutes.
  7. Rinse in distilled water.

What stain is used for reticular fibers?

Ammoniacal silver stains
Ammoniacal silver stains are the most commonly used methods for demonstration of reticular fibers.

What is a reticulin?

Reticulin is a histological term used to describe a type of fiber in connective tissue composed of type 3 collagen in which these reticular fibers crosslink to form a fine meshwork.

What is reticulin in bone marrow?

Reticulin is a normal component of the bone marrow stroma and can be detected with a reticulin stain in 73% to 81% of healthy subjects. 16-19. Increased reticulin staining (reticulin fibrosis) is associated with many benign conditions as well as some malignant diseases.

What are the special stains used in histopathology?

Hematoxylin and Eosin (H&E) staining is used routinely in histopathology laboratories as it provides the pathologist/researcher a very detailed view of the tissue. It achieves this by clearly staining cell structures including the cytoplasm, nucleus, and organelles and extra-cellular components.

What is the function of reticular fibers?

Reticular fibers crosslink to form a fine meshwork (reticulin). This network acts as a supporting mesh in soft tissues such as liver, bone marrow, and the tissues and organs of the lymphatic system.

Why are reticular fibers stained black?

Periodic acid-Schiff (PAS) reaction, which takes advantage of the high polysaccharide content of reticular fibers. Gomori and Wilder silver nitrate staining procedures, which make the fibers appear black (argyrophilic).

What is the principle of special staining?

In principle, they work by taking advantage of intra- and extra-cellular chemical reactions between the tissue components and dyes. Typically they use a chemical or dye with an affinity for whatever is under investigation, enabling specific tissues, structures, or even microorganisms to be stained.

Why do reticular Fibres appear as dark strands on a reticulin stained slide?

Where is reticulin found?

Reticulin fibers support the body and are common in the liver, spleen and kidneys.

What is trephine biopsy?

A trephine biopsy, sometimes done with a second needle, removes a small piece of bone with the marrow inside. The bone marrow samples are sent to a laboratory for examination under a microscope.