What is the principle of MTT assay?

Principle: Rapid colorimetric assay based on the cleavage of the tetrazolium ring of MTT (3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide) by dehydrogenases in active mitochondria of living cells as an estimate of viable cell number.

Why is DMSO used in MTT assay?

We have found that DMSO is the best solvent for dissolving the formazan product, especially where a significant amount of residual medium is left in the wells of the microtitre tray used for the assay.

Which drugs are cytotoxic?

Some examples of cytotoxic antibiotics include:

  • anthracyclines such as: doxorubicin (Lipodox) valrubicin (Valstar)
  • non-anthracyclines such as: bleomycin (Blenoxane) dactinomycin (Cosmegen)

Which drug has a cytotoxic activity?

A number of cytotoxic anti-cancer drugs including paclitaxel (Luo and Prestwich, 1999; Luo et al., 2000; Auzenne et al., 2007), doxorubicin (Luo et al., 2002), sodium butyrate (Coradini et al., 1999; 2004a,b), mitomycin c (Akima et al., 1996), and epirubicin (Akima et al., 1996) had increased CD44 specific …

What is cell viability and cytotoxicity?

Cell viability and cytotoxicity assays measure cellular or metabolic changes associated with viable or nonviable cells. These assays can detect structural changes such as loss of membrane integrity upon cell death or physiological and biochemical activities indicative of living cells.

Is MTT cytotoxic?

MTT assay is commonly used to assess the cellular cytotoxicity caused by anticancer drugs in glioblastomas. However, there have been some reports insisting that MTT assay exhibited non-specific intracellular reduction of tetrazolium which led to underestimated results of cytotoxicity.

Why is SDS used in MTT assay?

It has been known that several solvents show low cytotoxicity, and DMSO shows more cytotoxicity compare to ethanol (Klawitter et al., 2012). MTT assay with the use of 10% SDS solution in 0.01 M HCl as a formazan solvent is a convenient method to obtain reliable data.