What is EdU in staining?

In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample. A fluorescent azide, such as iFluor-488, is then added. The fluorescent azide is small enough to diffuse freely through native tissues and DNA, and it covalently cross-links to the EdU in a ‘click’ chemistry reaction.

How does EdU labeling work?

The results of our experiments indicate that EdU effectively labels cochlear supporting cells undergoing DNA synthesis during avian hair cell regeneration. The Click-iT™ technique is a simple, quick procedure that provides a very intense fluorescent label for proliferating cell nuclei that have incorporated EdU.

How is EdU detected?

Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA.

What does Ki67 stain?

Ki67 staining is frequently used in oncology to estimate a tumor’s proliferation index. Using immunohistochemistry of a biopsy, cells are scored as Ki67 positive or negative, and the biopsy is assigned a percent Ki67-positive value.

What is an LDH assay?

The LDH assay, also known as LDH release assay, is a cell death / cytotoxicity assay used to assess the level of plasma membrane damage in a cell population.

How do you dissolve EdU?

1. Prepare a 10 mg/mL solution of EdU by adding PBS to EdU powder and mixing well in a water bath (55°C-65°C) until the EdU is fully dissolved. Although the manufacturer recommends dissolving EdU powder in dimethylsulfoxide (DMSO), in our experience most of the tadpoles died after injection of EdU dissolved in DMSO.

Is EdU toxic?

Despite its many advantages, EdU is more toxic than BrdU, hindering its application for long-term labeling studies and perpetuating the search for improved DNA labeling tags.