What is the role of ammonium persulfate in preparing the polyacrylamide gels?

What is the role of ammonium persulfate in preparing the polyacrylamide gels?

Ammonium persulfate (APS) is an oxidizing agent that is often used with tetramethylethylenediamine (TEMED, Part No. 17919) to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

Which initiator is used in radical polymerisation?

Radical Chain-Growth Polymerization When radical polymerization is desired, it must be started by using a radical initiator, such as a peroxide or certain azo compounds.

What are the three steps of free radical polymerization?

Free Radical Polymerization occurs in three steps, i.e. Initiation, Propagation and Termination. Free Radical Polymerization is an essential technique for obtaining different polymers and material composites. It was used to get 40 billion of 110 billion polymers in the United States in 2001.

How does ammonium persulfate work?

The dissolution of the salt in water is an endothermic process. Ammonium persulfate acts as a polymerization initiator in polymer chemistry, as an etchant and cleaner in the manufacture of printed circuit boards, as a booster in hair bleaching formulations in cosmetics and as a gel breaker in the oil and gas industry.

What is the role of ammonium persulfate?

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism.

How does a radical initiator work?

In chemistry, radical initiators are substances that can produce radical species under mild conditions and promote radical reactions. These substances generally possess weak bonds—bonds that have small bond dissociation energies. Radical initiators are utilized in industrial processes such as polymer synthesis.

What are good radical initiators?

For example, azo compounds such as 2,2′-azobis(isobutyronitrile) (AIBN) and organic peroxides such as benzoyl peroxide (BPO) are well-known thermal radical initiators, and benzenesulfonic acid esters and alkylsulfonium salts have been developed as thermal cation initiators.

How does initiator work in polymerization?

initiator, a source of any chemical species that reacts with a monomer (single molecule that can form chemical bonds) to form an intermediate compound capable of linking successively with a large number of other monomers into a polymeric compound.

What is free radical initiator?

What is initiator in polymerization?

initiator, a source of any chemical species that reacts with a monomer (single molecule that can form chemical bonds) to form an intermediate compound capable of linking successively with a large number of other monomers into a polymeric compound. Related Topics: polymerization.