What is KANR in a plasmid?

The plasmid pKAN-R carries the kanamycin resistant gene, kanr, which encodes a phosphotransferase, an en- zyme that transfers a phosphate group to the kanamycin molecule destroying its antibiotic effects. Kanamycin is an antibiotic that kills bacteria by preventing them from making proteins.

Which is an example of plasmid vector?

Artificial Plasmid Vectors They have higher replication rates and are widely used in the field of genetic engineering. Examples – pBR322, pUC19 and pUC18.

What is autonomous plasmid?

Unlike conjugative plasmids that are extra-chromosomal and replicate autonomously, ICEs are integrated in the chromosome and replicate passively during chromosomal replication. It is generally thought that ICEs do not replicate autonomously.

What is a replication vector?

In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g., plasmid, cosmid, Lambda phages). A vector containing foreign DNA is termed recombinant DNA.

What is AmpR promoter?

AmpR promoter is the promoter for ampicillin resistance. It is a weak promoter. Any gene can also be cloned under this promoter and expressed in bacteria like E. Coli. There is a pCutamp plasmid used for plasmid-curing in Escherichia coli by targeting the AmpR promoter.

What are the different types of plasmid?

5 types of plasmids are given below.

  • Resistance Plasmids.
  • Virulence Plasmids.
  • Degradative Plasmids.
  • Col Plasmids.
  • Fertility F Plasmids.

What is plasmid give any two types?

Col plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. Degradative plasmids, which enable the digestion of unusual substances, e.g. toluene and salicylic acid. Virulence plasmids, which turn the bacterium into a pathogen. e.g. Ti plasmid in Agrobacterium tumefaciens.

What is autonomous replication of plasmid?

ARS, autonomously replicating sequences, are the sequences that confer on the plasmid the ability to replicate without integration into another replicon. From: Encyclopedia of Food Microbiology, 1999.

Are Col plasmids autonomously replicating?

INTRODUCTION. Plasmids are autonomously replicating extrachromosomal elements that facilitate molecular studies in bacteria as well as in yeasts and other fungi (1).

What is a plasmid cloning vector?

Plasmids are cloning vectors that are maintained in cells as autonomously replicating circular double-stranded DNA molecules. A great many cloning vectors that are in use today were derived from naturally occurring plasmids.