How is ATP affected by temperature?

Results. The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake.

At what temp does ATP synthase denature?

Above 40°C, the ATP-regenerating system was omitted because creatine kinase would be denatured.

Is ATP hydrolysis temperature dependent?

Temperature dependence of the hydrolysis activity. In the previous study (14), the ATP hydrolysis activity of the thermophilic F1 has been shown to be maximal at 75°C, with another peak at 20°C.

What is the relationship between temperature and metabolic rate?

As temperature increases, the rate of metabolism increases and then rapidly declines at higher temperatures – a response that can be described using a thermal performance curve (TPC).

What enzymes break down ATP?

ATPases are a group of enzymes that catalyze the hydrolysis of a phosphate bond in adenosine triphosphate (ATP) to form adenosine diphosphate (ADP).

Do enzymes bind to ATP?

ATP is proficient at interacting with other molecules through a binding site. The ATP binding site is the environment in which ATP catalytically actives the enzyme and, as a result, is hydrolyzed to ADP. The binding of ATP causes a conformational change to the enzyme it is interacting with.

What is the optimal pH for ATP synthase?

Maximal ATP synthase rates in membrane vesicles where E. coli F1FO is oriented with F1 on the outer surface are typically achieved with inner and outer pH values of 5.0 and 8.5, respectively (Fischer et al., 2000; Fischer et al., 1994).

What is the direct energy source that drives ATP synthesis during respiratory oxidative phosphorylation?

a difference of H concentration on opposite sides of the inner mitochondrial membrane.

Which enzyme catalyzes the hydrolysis of ATP?

Hydrolysis of ATP to adenosine diphosphate (ADP) and an inorganic phosphate group (Pi) is catalysed by the enzyme ATP hydrolase.

Which enzyme is responsible for the splitting of ATP?

…is produced by the enzyme ATP synthase, which converts ADP and phosphate to ATP. ATP synthase is located in the membrane of cellular structures called mitochondria; in plant cells, the enzyme also is found in chloroplasts. The central role of ATP in energy metabolism was discovered by Fritz Albert Lipmann…