What is a BAC library?

Bacterial artificial chromosome (BAC) libraries are the large DNA insert libraries of choice and valuable tools for the map-based cloning of target quantitative trait loci, physical mapping, molecular cytogenetics and comparative genomics.

How do you make a BAC library?

Making a BAC library To make a genomic Bacterial Artificial Chromosome (BAC) library you first have to isolate the cells containing the DNA you want to store. For animal and human BAC libraries the DNA normally comes from white blood cells. These isolated cells are then mixed with hot agarose, a jelly-like substance.

What are BACs used for?

A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism’s DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.

What is YAC library?

The yeast artificial chromosome (YAC) cloning system makes it possible to clone large pieces of genomic DNA into yeast. Libraries have been made containing clones with inserts in the megabase-pair range.

What is the BAC vector?

BAC vectors are plasmids constructed with the replication origin of E. coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to 300 kb. Since they are present in low copies, recombination between the high- copy plasmids.

What is BAC to BAC sequencing?

BAC to BAC Sequencing. The BAC to BAC approach first creates a crude physical map of the whole genome before sequencing the DNA. Constructing a map requires cutting the chromosomes into large pieces and figuring out the order of these big chunks of DNA before taking a closer look and sequencing all the fragments.

What are BAC vectors?

BAC vectors are plasmids constructed with the replication origin of E. coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to 300 kb.

What is BAC and YAC?

Description. YAC vectors are DNA constructs that are used for cloning DNA in yeasts. BAC vectors are DNA constructs that are used for cloning DNA in bacteria. Gene insert. A gene sequence of 100-1000 kbp can be inserted in these vectors.

What is a BAC vector?

What is BAC microarray?

A BAC clone chip is a DNA microarray whose probe is a template amplified by PCR. The template is a genome region incorporated into a comprehensive BAC (bacterial artificial chromosome) clone which various research institutions used in decoding the genome sequence of various organisms.

Why is BAC and AC used in gene cloning?

Hence, both YAC and BAC are artificial DNA constructs that can maintain large human and plant genomic fragments (> 300 kb) with a high degree of stability and thus, are used to construct genome libraries and in gene transfer.

What are BACs and YACS?

YAC vectors are DNA constructs that are used for cloning DNA in yeasts. BAC vectors are DNA constructs that are used for cloning DNA in bacteria. Gene insert. A gene sequence of 100-1000 kbp can be inserted in these vectors. A gene sequence of 150-350 kbp can be inserted into the BAC vectors.