Is edwardsiella tarda citrate positive?
Is edwardsiella tarda citrate positive?
Edwardsiella spp. usually are positive for lysine and ornithine decarboxylase activity, but they yield a negative Voges-Proskauer test result and do not use citrate as a sole carbon source. Most clinical isolates of E. tarda produce hydrogen sulfide on Salmonella-Shigella agar plates and do not ferment lactose.
Is edwardsiella urease positive?
It is positive for glucose fermentation, but negative for lactose fermentation and is unable to grow on D-mannitol or D-sorbitol. E. tarda is also oxidase-negative and catalase-positive. It does not produce urease but is similar to Salmonella in that it is able to generate hydrogen sulfide on laboratory media.
What biochemical tests are used to identify Staphylococcus?
3.1. Biochemical tests used to confirm S. aureus were coagulase test, catalase test, indole production, methyl red test, Voges-proskauer reaction, urease production, citrate utilization and sugar fermentation [12].
Is edwardsiella tarda lysine decarboxylation positive?
Edwardsiella generally are positive for both lysine and ornithine decarboxylase activity, but yield a negative Voges–Proskauer test result and do not use citrate as a sole carbon source.
Does edwardsiella tarda produce gas?
are biochemically similar to E. coli; however, Edwardsiella tarda has the distinction of producing hydrogen sulfide; it can cause gastroenteritis and infect open wounds in humans.
Is edwardsiella motile or nonmotile?
It is motile, and is catalase and indole positive. This organism is more often isolated from patients with gastrointestinal disease than form healthy individuals Muller (1986). It has been reported to cause neonatal sepsis, as well as brain and tubo-ovarian abscesses.
What is the confirmatory test for Staphylococcus aureus?
tube coagulase test
In many developing countries, the tube coagulase test is usually confirmatory for S. aureus and is routinely done using either human or sheep plasma. This study evaluated Mannitol salt agar and the deoxyribonuclease (DNase) test for improving the efficiency of the tube coagulase test in resource limited settings.
What tests can you use to distinguish Staphylococcus aureus from Staphylococcus epidermidis?
Staphylococcus aureus produces free coagulase; Staphylococcus epidermidis does not. The coagulase test is useful for differentiating potentially pathogenic Staphylococci such as Staphylococcus aureus from other Gram positive, catalase-positive cocci.
Which test is used for identification of Enterobacteriaceae?
Biochemical tests, for example Beta-glucoronidase test, citrate utilization test, urease test, indole test, are the basic tests used for Enterobacteriaceae identification in Nigerian hospitals (Cheesebrough, 2000).
What does edwardsiella tarda cause?
Edwardsiella tarda is a well-known cause of haemorrhagic septicaemia in fish and has been associated with gastro- and extraintestinal infections in humans (Jaruratanasirikul and Kalnauwakul, 1991; Janda et al., 1991; Janda and Abbott, 1993, 1999; Srinivasa-Rao et al., 2004).
How is edwardsiella treated?
FIRST AID/TREATMENT: Gastroenteritis caused by E. tarda resolves spontaneously without any antibiotic therapy 3. Aggressive cases can, however, be treated with amoxicillin (e.g. 80 mg/kg twice daily for 3 days), cotrimoxazole, and cephalosporins 3. Extraintestinal infections due to E.