What is the colony morphology of Staphylococcus aureus?

S. aureus is a facultatively anaerobic, Gram-positive coccus, which appears as grape-like clusters when viewed through a microscope, and has round, usually golden-yellow colonies, often with hemolysis, when grown on blood agar plates.

What is the colony colour of Staphylococcus aureus?

S. aureus produces the yellow pigment staphyloxanthin and characteristic gold-colored colonies are formed on all rich media including tryptic soy agar (TSA) at 37°C, brain heart infusion (BHI) agar and Luria Bertani (LB) agar.

What are the three identifying characteristics of S. aureus?

Answer and Explanation: Staphylococcus aureus is a coagulase-positive, gram-positive coccus in grape-like clusters, salt-tolerant on mannitol salt agar, and part of the norma… See full answer below.

How do you identify Staphylococcus aureus in a lab?

Coagulase testing is the single most reliable method for identifying Staphylococcus aureus [9]. Coagulase production can be detected using either the slide coagulase test (SCT) or the tube coagulase test (TCT).

How can you describe the growth colonies of staph aureus in blood agar plates?

On blood agar plates, colonies of Staphylococcus aureus are frequently surrounded by zones of clear beta-hemolysis. The golden appearance of colonies of some strains is the etymological root of the bacteria’s name; aureus meaning “golden” in Latin.

What is the morphology of Staphylococcus?

Morphology: Spherical cocci; often forming regular and “grape-like” clusters. They occur singly, in pairs, tetrads, and short chains.

Does S. aureus grow on blood agar?

S. aureus appears as grape-like clusters when viewed through a microscope, and has large, round, golden-yellow colonies, often with beta hemolysis, when grown on blood agar plates.

How do you identify a Staphylococcus aureus culture?

Two different coagulase tests are commonly used to identify S. aureus. One is a tube test for free coagulase and the other is a slide test for bound coagulase. The tube coagulase test is thought to be the more definitive of the two, however, it can take several hours to overnight to produce a result.

What color is Staphylococcus aureus after Gram staining?

purple
After gram staining, gram-positive Staphylococcus aureus turn purple and gram-negative Escherichia coli turn pink. After gram staining, gram-positive Staphylococcus aureus turn purple and gram-negative Escherichia coli turn pink.

What agar does Staphylococcus aureus grow on?

Staph. aureus will grow on general culture media such as Blood Agar and chocolated Blood Agar and therefore can be isolated from direct plating of clinical specimens.

What Agar does Staphylococcus aureus grow on?